Nathalie FISHER-DURAND

Research engineer

Sorbonne University
4 Place Jussieu, 75252 Paris cedex 5
Tower 33-43, 4th floor
CHEMBIO Group
Phone: 33(0)1 44 27 67 32

Research interests

Protein chemistry, dendrimer chemistry, bioanalysis using metal-carbonyl complexes.

Current research

  • Development of bioorthogonal crosslinkers for bioconjugation.
  • Polyfunctionalization of PAMAM dendrimers to access multimodal platforms of diagnostic or therapeutic interest.
  • Quantitative analysis by infrared using metal-carbonyl complexes.
  • Labelling of proteins and antibodies with metal-carbonyl or fluorescent probes for analytical applications.

Selected recent results

  • Labelling of BSA (Bovine Serum Albumine) with metal carbonyl complexes as infrared probes by click chemistry (CuAAC and SPAAC) (ref 2) : 
  • Functionalization of PAMAM dendrimer with metal carbonyl infrared probe and azide handle (ref 2):
  • Synthesis of a new bioorthogonal and heterobifunctional crosslinker for the labelling of biomolecules :

Scientific career

  • Chemistry studies at University Paris-Sud (Orsay).
  • 1988-1991 : Ph. D. thesis in organic chemistry (University Paris-Sud) under the supervision of Dr. Claude Wakselman (CNRS-CERCOA, Thiais) : Synthesis of fluorinated analogs of key metabolites of the shikimate pathway.
  • 1992-1994 : Post-doctoral research associate at the Faculty of Pharmaceutical Sciences in Châtenay-Malabry (University Paris-Sud) under the supervision of Dr. Jean-Pierre Bégué : Synthesis of potential inhibitors of the HIV-1 protease.
  • 1995-1999 : Post-doctoral research associate at Chimie ParisTech (Ecole Nationale Supérieure de Chimie de Paris) under the supervision of Prof. François Le Goffic : Development of immunosorbents for the analysis of traces of pesticides in the environment.
  • Since 1999 : Engineer position at the CNRS (Chimie ParisTech (1999-2013) in the team of Dr. Anne Vessières), and at Sorbonne University – Paris VI since 2014, in the ChemBio team.

Selected publications

  1. A new bioorthogonal cross-linker with alkyne and hydrazide end groups for chemoselective ligation. Application to antibody labelling. Fischer-Durand, N. ; Salmain, M. ; Vessières, A. ; Jaouen, G.,. Tetrahedron 2012, 68, 9638-9644.
  2. ‘Clickable’ cyclopentadienyl iron carbonyl complexes for bioorthogonal conjugation of mid‐infrared labels to a model protein and PAMAM dendrimer. Fischer‐Durand, N. ; Lizinska, D. ; Guérineau, V. ; Rudolf, B. ; Salmain, M. ; Appl. Organomet. Chem. 2019 ; e4798.
  3. Inverse electron-demand Diels-Alder (iEDDA) bioorthogonal conjugation of half-sandwich transition metallocarbonyl entities to a model protein. Jamroz, D. ; Fischer-Durand, N. ; Palusiak, M. ; Wojtulewski, S. ; Jarzynski, S., Stepniewska, M. ; Salmain, M. ; Rudolf, B. ; Appl. Organomet. Chem. 2020 ; e5507.

List of publications in Hal