Size-exclusion chromatography (SEC)

This characterization method enables the separation of macromolecules according to their hydrodynamic volume. This separation takes place through columns filled with a gel composed of porous beads. The size of the pores in this gel determines the range of molar masses (MW) that can be separated by the system. Depending on the detectors present at the column outlets, it is then possible to determine the average molar masses (MnMw), dispersity (Đ) and size (Rg, Rh) or architecture of the macromolecules. The ECP laboratory has 5 SEC systems :

Malvern Panalytical

  • Solvent : THF (40°C)
  • Detectors: Refractometer / UV
  • MW separation range:
    200 – 2 000 000 g/mol

Malvern Panalytical

  • Solvent : THF (40°C)
  • Detectors: Refractometer / UV / light scattering (2 angles) / viscosimeter
  • MW separation range:
    200 – 2 000 000 g/mol

Malvern Panalytical

  • Solvent : DMF + LiBr 1g/L (60°C)
  • Détecteurs: Refractometer / UV / light scattering (2 angles) / viscosimeter
  • MW separation range:
    100 – 1 000 000 g/mol

Malvern Panalytical

  • Solvent : DMSO + LiBr 1g/L (80°C)
  • Detectors: Refractometer
  • MW separation range:
    100 – 1 000 000 g/mol

Agilent

  • Solvent : THF (20°C)
  • Detectors: Refractometer / UV
  • MW separation range:
    500 – 60 000 g/mol

CONTACT :

Gaëlle PEMBOUONG

Engineer

Corridor 43-53, office 408
POLYMER team
gaelle.pembouong[at]sorbonne-universite.fr