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Research Engineer, PhD

Sorbonne Université,
4 Place Jussieu, 75252 Paris cedex 5

Couloir 33-43 4ème étage. case 229
Team Chembio

Tel:33(0)1 44 27 67 32


Research interests:

Protein chemistry, dendrimer chemistry, bioanalysis using metal-carbonyl complexes.

Current research:

  • Development of bioorthogonal crosslinkers for bioconjugation.
  • Polyfunctionalization of PAMAM dendrimers to access multimodal platforms of diagnostic or therapeutic interest.
  • Quantitative analysis by infrared using metal-carbonyl complexes.
  • Labelling of proteins and antibodies with metal-carbonyl or fluorescent probes for analytical applications.

Recent results :

  • Labelling of BSA (Bovine Serum Albumine) with metal carbonyl complexes as infrared probes by click chemistry (CuAAC and SPAAC) (ref 2) :
  • Functionalization of PAMAM dendrimer with metal carbonyl infrared probe and azide handle (ref 2):
  • Synthesis of a new bioorthogonal and heterobifunctional crosslinker for the labelling of biomolecules :

Scientific career

  • Chemistry studies at University Paris-Sud (Orsay).
  • 1988-1991 : Ph. D. thesis in organic chemistry (University Paris-Sud) under the supervision of Dr. Claude Wakselman (CNRS-CERCOA, Thiais) : Synthesis of fluorinated analogs of key metabolites of the shikimate pathway.
  • 1992-1994 : Post-doctoral research associate at the Faculty of Pharmaceutical Sciences in Châtenay-Malabry (University Paris-Sud) under the supervision of Dr. Jean-Pierre Bégué : Synthesis of potential inhibitors of the HIV-1 protease.
  • 1995-1999 : Post-doctoral research associate at Chimie ParisTech (Ecole Nationale Supérieure de Chimie de Paris) under the supervision of Prof. François Le Goffic : Development of immunosorbents for the analysis of traces of pesticides in the environment.
  • Since 1999 : Engineer position at the CNRS (Chimie ParisTech (1999-2013) in the team of Dr. Anne Vessières), and at Sorbonne University - Paris VI since 2014, in the ChemBio team.

Selected publications:

  1. A new bioorthogonal cross-linker with alkyne and hydrazide end groups for chemoselective ligation. Application to antibody labelling. Fischer-Durand, N.; Salmain, M.; Vessières, A.; Jaouen, G.,. Tetrahedron 2012, 68, 9638-9644.
  2. ‘Clickable’ cyclopentadienyl iron carbonyl complexes for bioorthogonal conjugation of mid‐infrared labels to a model protein and PAMAM dendrimer. Fischer‐Durand, N. ; Lizinska, D. ; Guérineau, V. ; Rudolf, B. ; Salmain, M. ; Appl. Organomet. Chem. 2019; e4798.
  3. Inverse electron-demand Diels-Alder (iEDDA) bioorthogonal conjugation of half-sandwich transition metallocarbonyl entities to a model protein. Jamroz, D.; Fischer-Durand, N.; Palusiak, M.; Wojtulewski, S.; Jarzynski, S., Stepniewska, M.; Salmain, M.; Rudolf, B.; Appl. Organomet. Chem. 2020 ; e5507.